FIG 2.

Transfection of cells with dominant negative dynamin inhibits HSV infection of neuronal cells. SK-N-SH cells were transfected with a plasmid expressing the dominant negative form of dynamin 1 (K44Adnm1) or dynamin 2 (K44Adnm2) or with a control GFP-expressing plasmid (pmaxGFP). Twenty-four hours later, the cells were infected with HSV-1(F-GS2822) (MOI, 10), which encodes the red fluorescent protein fused to the N terminus of VP26. Cells were fixed 16 h p.i., and nuclei were stained with DAPI (blue). (A) Representative images from two independent experiments. (B) Proportions of transfected, uninfected (green), transfected, infected (yellow), nontransfected, uninfected (white), and nontransfected, infected (red) cells, quantified after a review of 5 independent fields (total of 100 cells). (Left) pmaxGFP; (center) K44Adnm1; (right) K44Adnm2.