FIG 4.

Dynasore inhibits HSV infection postentry. (A) SK-N-SH, CaSki, or primary human female genital tract cells isolated from cervicovaginal lavage fluid were first infected with HSV-2(G) for 1 h at 37°C, then washed, and finally overlaid with fresh medium. Dynasore was added either during the 1-h entry period, followed by washing of the cells (0 h postentry), or 1, 4, or 6 h postentry. Plaques were counted 24 h (for SK-N-SH cells) or 48 h (for CaSki and primary genital tract cells) p.i. (B) SK-N-SH cells were infected with HSV-2(G), and ML141 (10 or 100 μM) either was added 1 or 4 h postentry or was maintained in the cultures throughout the infection. Results are presented as the percentages of reduction in PFU from the level for cells treated with a control buffer (0.25% DMSO in serum-free medium). Asterisks indicate significance (P < 0.001) by t test.