FIG 5.

Dynasore has potent postentry effects on HSV infection of primary fetal neuronal cells. Fetal neuronal cells were infected with the GFP-expressing virus HSV-2(333ZAG) (MOI, 0.5 PFU/cell). After 1 h of incubation, the cells were treated with a citrate buffer to inactivate any virus that had not yet penetrated them, washed, and overlaid with fresh medium. Dynasore or a 0.25% DMSO control buffer was either added once, at the time of infection (t = 0), immediately after citrate treatment (t = 1), or 4 h after citrate treatment (t = 4), or was added both at the time of infection and after citrate treatment (throughout). (A) Representative fluorescence microscopy images of live cells 48 h postinfection. (B) Percentages of cells that were infected (GFP positive), quantified after review of several independent fields (500 to 600 cells).