FIG 4.

Germination of Δsap8 and SAP6 OE cells resulted in formation of large cell-cell aggregates. Cells were grown at 37°C for 3 h to form hyphae and observed under ×20 magnification. (Top row) No aggregation was found in yeast form cells of the CAI4, Δsap8, SAP5 OE, and SAP6 OE strains. (Middle and bottom rows) Hypha formation was accompanied by the formation of large aggregates in the Δsap8 and SAP6 OE strains, but not in the wild-type CAI4 strain, while the SAP5 OE strain formed smaller aggregates. The boxed areas in the middle row are shown at higher magnification in the bottom row.