TABLE 2.

Expression profile of secreted aspartyl proteinase genes from tongue plaques or agar surfaces

Origin	Strain	Expression levela
		SAP1	SAP2	SAP3	SAP4	SAP5	SAP6	SAP7	SAP8	SAP9	SAP10
Tongue plaque	WT	0.05 ± 0.01	1.25 ± 0.05	0.12 ± 0.02	2.07 ± 0.51	5.32 ± 2.03	9.14 ± 2.83	0.33 ± 0.02	0.43 ± 0.02	0.16 ± 0.08	0.19 ± 0.07
	Δsap8	0.11 ± 0.03	1.43 ± 0.70	0.12 ± 0.01b	3.74 ± 1.08	12.29 ± 1.95c	38.21 ± 5.08d	0.18 ± 0.07	ND	0.05 ± 0.02	0.18 ± 0.04
Agar surface	WT	3.64 ± 1.08	4.44 ± 1.05	2.63 ± 0.96	2.24 ± 0.63	12.72 ± 2.36	21.34 ± 4.49	4.31 ± 1.34	0.14 ± 0.09	4.30 ± 0.94	1.64 ± 0.03
	Δsap8	1.16 ± 0.16b	2.38 ± 0.32	0.21 ± 0.04b	2.04 ± 0.21	22.90 ± 5.59c	63.79 ± 6.44d	0.43 ± 0.10c	ND	1.24 ± 0.58c	2.38 ± 0.35

^aGene expression levels were calculated as a ratio to targeted gene/ACT1 expression calculated from a cDNA standard curve. Differences in expression values between the WT and Δsap8 strains for each gene and each growth condition were measured using Student's t test. ND, not determined.

^bP < 0.05.

^cP < 0.01.

^dP < 0.001.