FIG 4.

C. albicans gpi7 mutant could be recognized by host macrophages. gpi7Δ/Δ could induce NF-κB and MAPK activation and inflammatory responses in macrophages. Thioglycolate-elicited peritoneal macrophages were stimulated by UV-inactivated C. albicans yeast SN152, gpi7Δ/Δ::GPI7, and gpi7Δ/Δ (MOI of 5) for the indicated times. The nuclear extracts (A) and cell lysates (B and C) were analyzed by immunoblotting with the indicated antibodies. (D) Enzyme-linked immunosorbent assay (ELISA) results for TNF-α, IL-6, IL-12p40, and IL-10 in supernatants of thioglycolate-elicited peritoneal macrophages, which were stimulated with the indicated C. albicans strains (MOI of 5) for 6 h. Mock, unstimulated macrophages. Data represent means (± SD) from triplicates of one representative experiment of three. *, P < 0.05 (one-way ANOVA with Bonferroni's posttest).