FIG 6.
Host increased inflammatory responses in vivo against C. albicans gpi7Δ/Δ. C57BL/6 or Dectin-1-deficient mice were intraperitoneally infected with 2 × 105 UV-inactivated C. albicans organisms of SN152, gpi7Δ/Δ::GPI7, or gpi7Δ/Δ for 4 h. (A) Flow cytometry for Gr-1hi7/4hi neutrophils and Gr-1+7/4hi F4/80+ inflammatory monocytes in the peritoneum. (B) Scatter plots of myeloid cell subsets in the peritoneum. Each symbol represents an individual mouse. Neut, neutrophil; Mono, monocyte; Eos, eosinophil. (C) ELISAs for cytokines, chemokines, and growth factors in lavage fluid from the peritoneal cavities. MCP-1, chemokine CCL2; GM-CSF, granulocyte-monocyte colony-stimulating factor. Data are representative of three independent experiments. P < 0.05 (*) and P < 0.01 (**) (Mann-Whitney nonparametric t test [B] and Kruskal-Wallis nonparametric one-way ANOVA with Dunn's posttest [C]).