FIG 1.
Production of enzymatically inactive SsES178A by MGAS5005 Δsse-sseS178A. (A) Western blots showing that MGAS5005 Δsse-sseS178A had similar levels of SsES178A in its culture supernatant as the positive-control strains. Blots for the M protein and Spy19 are included as controls. Lane 1, MGAS5005; lane 2, MGAS5005 Δsse; lane 3, MGAS5005 Δsse-sse; lane 4, MGAS5005 Δsse-sseS178A. (B) PAF acetylhydrolase (AH) activity measured by the 2-thio-PAF acetylhydrolase assay in 10 μl of culture supernatant of MGAS5005, MGAS5005 Δsse, MGAS5005 Δsse-sse, and MGAS5005 Δsse-sseS178A.