FIG 1.

Measurement of S1P levels after infection of mice or an alveolar macrophage cell line with C. neoformans (Cn). (A to C) S1P levels in the BAL fluid of CBA/J, Tgε26, C57BL6/J, and SK1^−/− mice uninfected or infected with the C. neoformans H99 WT or C. neoformans Δgcs1 strain. Data were collected from three mice per time point at 4 (A), 8 (B), and 16 (C) days postinfection. (D) S1P levels in the medium of the MHS macrophage cell line 2 h after infection with C. neoformans (MHS/C. neoformans ratio = 1:10). *, values significantly (P < 0.01) greater than those for both uninfected mice and mice infected with the C. neoformans H99 WT strain.