FIG 8.

S. marcescens strain K904 produces more PrtS than SlpB. (A) PAGE analysis of secretomes of K904 and noted isogenic mutants. Secretomes were normalized by optical density and trichloroacetic acid precipitated, and equal volumes were loaded. The large black arrow indicates an ∼50-kDa band. The small black arrow indicates SlpB. The small white arrow indicates PrtS. (B) Quantification of the 50-kDa band from 4 gels using independent samples. Values were normalized to the 50-kDa band from the K904 secretomes. Means and standard deviations are shown. The asterisk equals significant difference from K904 by ANOVA with Tukey's posttest.