FIG 2.

Multiplication of diverse WT and hmgb2-deficient parasites according to the host mouse strain. (A and B) Survival (A) and parasitemia (B) of BALB/c mice infected with WT P. berghei ANKA and supplemented or not from days 4 to 8 p.i. (dashed line) with either recombinant PbHMGB2 (25 mg/kg) or vehicle (protein buffer plus 50 ng LPS, which takes into account the residual LPS contamination of the recombinant protein) twice a day (every 12 h). All mice were injected i.v. with 10⁶ red blood cells infected with WT P. berghei ANKA. Differences in mortality/survival between WT-infected mice, supplemented or not with PbHMGB2, were analyzed by the log rank test. (C and D) Survival (C) and parasitemia (D) of C57BL/6 mice infected with WT P. berghei NK65 and supplemented with recombinant PbHMGB2 as described above. All mice were injected i.v. with 10⁵ red blood cells infected with WT P. berghei NK65. For panels A to D, groups of mice contained five mice each. (E and F) Red blood cell development of P. berghei ANKA (E) and P. berghei NK65 (F), as well as their Δhmgb2 counterparts, in infected BALB/c and C57BL/6 mice, respectively. Parasitemia was measured by flow cytometry (E) and Giemsa counting (F) for sets of five mice. Values represent means ± standard deviations (SD) for one representative experiment of three. Statistical analyses of parasitemia were performed by the Student t test.