FIG 6.

Reduced parasite biomass in the organs of Δhmgb2 ANKA-infected mice. At d6 postinfection, RNAs were extracted from the brains (A), lungs (B), and spleens (C) of WT- or Δhmgb2 ANKA-infected C57BL/6 mice (10⁵ infected erythrocytes per mouse). Bars represent the mean ± SD parasite loads as evaluated by relative quantification of the 18S mRNA expression level, normalized via the hypoxanthyl-guanine phosphoribosyl transferase (hprt) transcript. Gray bars, WT P. berghei ANKA-infected mice; black bars, Δhmgb2 ANKA-infected mice. (D) Parasitemia was measured by counting after Giemsa staining. Values represent means ± SD. Comparisons of parasite biomass and parasitemia were analyzed by the Student t test. **, P < 0.01; ***, P < 0.001.