FIG 8.

FPKM values of genes within the Ysa locus under each experimental condition. Pooled FPKM values from three replicates for each gene within the Ysa locus were plotted along the Ysa locus after 120 min of infection under each experimental condition. Genes depicted in blue encode proteins that make up the Ysa T3S apparatus, genes depicted in white encode chaperones, genes depicted in black encode translocon proteins, genes depicted in green encode regulators of the system, and the red gene encodes a secreted effector of the system. Note the general increase in expression across the locus in the intracellular condition.