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The IgG antibody response levels in rats immunized with SAGs were determined by indirect ELISA. Sera were obtained from rats in groups A (nonimmunized control), B (orally immunized), C (subcutaneously immunized), and D (intravenously immunized) at weeks 2, 4, 6, and 9. Results are expressed as means ± the standard errors of the means. The asterisks indicate significant differences between the antibody responses of the immunized and nonimmunized groups (**, P < 0.01; *, P < 0.05).
