FIG 2.

Transcription of C. muridarum PZ ORFs initiates in the early to middle phase of the developmental cycle. Total RNA was isolated from C. muridarum-infected McCoy cells at 0, 6, 12, 18, 24, and 30 h p.i. (A) RT-PCR at 24 h p.i. indicated that all genes in the PZ are transcribed. Whether amplification was performed with (+) or without (−) reverse transcriptase is indicated. (B) The kinetics of transcription of select PZ ORFs was characterized by qRT-PCR. Transcript levels were normalized to standard curves of dilutions of C. muridarum chromosomes. The data represent the mean transcript levels with standard deviations (SD) from three independent experiments. euo, ompA, and omcB represent early, mid-late, and late genes, respectively.