TABLE 2.
Cell morphology and the level of 8-oxo guanine in H. pylori cells
| Strain | % of damaged cellsa | 8-oxoG level (FITC/PI intensity ratio)b |
|---|---|---|
| Wild type | ∼10 | 0.86 + 0.05 |
| trxA mutant | ∼45 | 1.45 + 0.06 |
| trxC mutant | ∼85 | 2.05 + 0.13 |
a
Cell morphology was examined by fluorescence microscopy, and the numbers are estimated percentages of damaged cells, including coccoid and broken cells, in the whole-cell population.
b
The 8-oxoG level is expressed as the mean intensity ratio of FITC to PI, with the standard deviation. According to statistical analysis with Student's t test, the data for each mutant strain are significantly different from those for the wild type (P < 0.001).