FIG 1.
Schematic of the AM-19226 T3SS genomic island. The AM-19226 T3SS genomic island is composed of a conserved “core” region that is similar among T3SS-positive Vibrio strains and mosaic 5′- and 3′-flanking regions that differ among clades and species. Genes encoding the T3SS structural proteins (VcsRTCNS2, VcsVUQ2, VcsJ2, and VspD) are shown as dark gray arrows, genes encoding the translocated proteins (Vops) are shown as checkered arrows, genes encoding the T3SS transcriptional regulators (VttRA and VttRB) are shown as black arrows, and genes encoding predicted virulence-related proteins (Acfs and TDH-related hemolysin [TRH]) are shown as light gray arrows. Note that genes for 5 of the 12 translocated proteins are interspersed within the four structural gene operons in the conserved core region, which also includes VopF. Arrows indicate the major transcriptional initiation sites based on transcriptome sequencing data (16).