Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
ΔvcsN2 and ΔvopA strains are deficient in adherence to Caco2-BBE cells. Caco2-BBE cells were grown to confluence on collagen-coated coverslips and cocultured for 2 h with either the AM-19226 parental strain or strains deleted for vcsN2 or vopA, after which nonadherent bacteria were washed away. After Triton X-100 treatment, the resulting lysates were serially diluted and plated onto selective medium to determine the number of adhered bacteria. *, P < 0.05.
