FIG 4.

In vitro polarization and characterization of BMDM from wild-type (WT) or 5-LOX^−/− mice. Following treatment with polarizing cytokines M(LPS+IFN-γ) or M(IL-4) for 24 h, supernatant nitrite (A) and cell lysate arginase activity (B) were determined. Cells were gated on live cells and F4/80⁺ and then assessed for percentage of expression of NOS2 (C), CD206 (D), or both NOS2 and CD206 (E) by flow cytometry. Asterisks indicate statistically significant differences between 5-LOX cells compared with WT controls from the same group at a P value of >0.05.