TABLE 1.
Strains and plasmids
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Strains | ||
| CF74 | C. freundii isolate from fecal samples from a goat | Laboratory stock |
| CF74ΔT6SS | CF74 mutant with T6SS core component gene cluster deletion | This study |
| CF74ΔclpV | CF74 mutant with clpV gene deletion | This study |
| CF74Δhcp-2 | CF74 mutant with hcp-2 gene deletion | This study |
| CF74Δvgr | CF74 mutant with vgr gene deletion | This study |
| E. coli SM10λpir | supE recA::rp4-2-Tc::Mu Kmr λpir | Laboratory stock |
| E. coli DH5αλpir | RP4-2-tet Mu-Km::Tn7 integrant leu-63::IS10 recA1 creC510 hsdR17 endA1 zbf-5 uidA(ΔmluI)::pir+ thi | Laboratory stock |
| CF74ΔclpV(pBAD24-clpV) | ΔclpV(pBAD24-clpV) | This study |
| CF74Δhcp-2(pBAD24-hcp-2) | Δhcp-2(pBAD24-hcp-2) | This study |
| CF74Δvgr(pBAD24-vgr) | Δvgr(pBAD24-vgr) | This study |
| Plasmids | ||
| pBAD24 | pMB1 Ampr; pBAD promoter | Laboratory stock |
| pBAD24-clpV | The clpV coding region was cloned into the EcoRI and HindIII sites of pBAD24 | This study |
| pBAD24-hcp-2 | The hcp-2 coding region was cloned into the KpnI and HindIII sites of pBAD24 | This study |
| pBAD24-vgr | The vgr coding region was cloned into the KpnI and HindIII sites of pBAD24 | This study |
| pWM91 | Ampr f1+ ori lacZa of pBluescript II SK(+); oriRR6Kγ oriTRP4 sacB Sucs | Laboratory stock |
| pWM91ΔT6SS | pWM91-T6SSup::T6SSdown; Ampr | This study |
| pWM91ΔclpV | pWM91-clpVup::clpvdown; Ampr | This study |
| pWM91Δhcp-2 | pWM91-hcp-2up::hcpdown; Ampr | This study |
| pWM91Δvgr | pWM91-vgrup::vgrdown; Ampr | This study |