FIG 6.

GelE from E. faecalis V583 induces permeability in epithelial cell monolayers. Caco-2 (A) and T-84 (B) cell monolayers were incubated with GelE purified from E. faecalis V583 (filled bars). The flux of FITC-dextran across monolayers (indicative of paracellular permeability) was significantly greater when monolayers were apically (Caco-2) or basally (T-84) cultured with GelE from E. faecalis V583 for 24 h than when monolayers were cultured with the medium control (open bars) (*, P < 0.0001). Each experimental group represents at least 3 independent experiments with 2 technical replicates in each experiment. Data are expressed as mean values ± standard errors. Comparisons were made using the nonparametric Mann-Whitney test.