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. 2015 Jun 16;5:10885. doi: 10.1038/srep10885

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Copyright © 2015, Macmillan Publishers Limited

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RNAi of BmADC. (a) The temporal expression pattern of BmADC from the wandering to moth stages. W0-W60 represents 0, 12, 24, 36, 48, and 60 h of the wandering stage, respectively. P1-P10 represents days 1 to 10 after pupation, respectively. M0 represents the first day of the moth stage. BmActin3 gene was used as an internal control for RT-PCR. (b) Phenotype of pupae at day 1 of pupation after RNAi. The black and red arrows point to the melanic and dark areas in the dsBmADC injected individuals, respectively. Scale bar: 1 cm. (c) Relative expression levels of BmADC at day 1 of pupation in individuals subjected to RNAi (n = 3; dsRed as a control; Student’s t-test, **, p < 0.01). Data are presented as means ± SD.