Figure 3.

Effect of alisertib and TAK-733 alone and in combination on the cell cycle. Left: Cell cycle analysis of double-mutant CRC cell lines upon exposure to alisertib and TAK-733 as single agents and in combination for 24 h by flow cytometry, with more pronounced G2/M delay observed in HCT116 and DLD-1 cell lines. Right: Immunoblotting for cyclin B1 and phosphorylated cyclin B1 in double-mutant cell lines, with increase in cyclin B1 observed upon exposure to alisertib alone for 24 h, and further when combined with TAK-733 for 24 h, in 3 of 4 cell lines.