Fig. 1.

SR1555 impacts adipogenic markers. (A) Chemical structure of SR1555 and (B) gene expression analysis in differentiated adipocytes. 3T3-L1 cells were grown to confluence, and then incubated with dexamethasone, insulin, and isobutylmethylxanthine for 2 days to induce differentiation. Cells were then treated with 20 μM SR1555 and insulin for 5 days, after which the expression of thermogenic genes and several nuclear receptors were analyzed by quantitative polymerase chain reaction. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression was used for normalization. Values are the mean ± S.E.M. of three samples per group. *P < 0.05, ***P < 0.001 versus dimethylsulfoxide (DMSO), as determined using Student’s unpaired t test.