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. 2015 Jul;88(1):75–83. doi: 10.1124/mol.114.096776

Fig. 5.

Fig. 5.

Functional characteristics of an OCT3 3′UTR variant (rs2076828) and its association with metformin pharmacologic response in healthy volunteers. The OCT3 3′UTR variant (C>G) in healthy human volunteers is associated with changes in metformin pharmacodynamics. (A) Luciferase reporter assay of rs2076828. Luciferase activity was significantly lower in HCT-116, DU145, and A549 cell lines transfected with a reporter construct containing the minor allele (G) compared with the reference allele (C). Data represent mean ± S.D., n = 3 replicates per cell line, *P < 0.05 and **P < 0.01. (B) E-QTL analysis of rs2076828 in healthy human cohorts revealed lower mRNA level of OCT3 in adipose tissues of subjects with the minor allele (G), P < 0.01. (C) The change in glucose exposure after an oral glucose tolerance test (AUC) after metformin treatment in healthy volunteers. Change in glucose AUC was calculated as glucose AUC after metformin dosing minus glucose AUC before metformin dosing. Healthy volunteers with the minor allele had lower response to metformin (P < 0.05). The dot plot displays the mean ± S.D.