TABLE 2.
HPLC analysis of muropeptides prepared from the peptidoglycan of the different mutants
| Strain or mutant | Relative abundance (mol%) of muropeptidea: |
Cross-linkb | D-D/Tc | Peptidoglycan lengthd | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mono | Di | Tri | D-D | Lpp | Anh | Penta | ||||
| PAO1 | 57.8 | 38.3 | 3.9 | 1.9 | 3.1 | 8.7 | 1.6 | 46.1 | 4.1 | 11.5 |
| PAO ΔdacB | 56.1 (1.0) | 39 (1.0) | 4.8 (1.2) | 1.5 (0.8) | 3.7 (1.2) | 9.6 (1.1) | 2.4 (1.5) | 48.9 (1.1) | 3.1 (0.8) | 10.5 (0.9) |
| PAO ΔdacC | 59.3 (1.0) | 37.1 (1.0) | 3.6 (0.9) | 1.1 (0.6) | 3.2 (1.0) | 9.1 (1.1) | 7.1 (4.4) | 44.4 (1.0) | 2.4 (0.6) | 11 (1.0) |
| PAO ΔpbpG | 58.7 (1.0) | 37.2 (1.0) | 4.1 (1.1) | 1.5 (0.8) | 2.8 (0.9) | 9.4 (1.1) | 1.4 (1.0) | 45.4 (1.0) | 3.2 (0.8) | 10.7 (0.9) |
| PAO ΔdacB ΔdacC | 58.4 (1.0) | 36.7 (1.0) | 4.8 (1.2) | 1.1 (0.6) | 3.4 (1.1) | 9.4 (1.1) | 28.6 (17.9) | 46.6 (1.0) | 2.3 (0.6) | 10.7 (0.9) |
| PAO ΔdacB ΔpbpG | 54.5 (0.9) | 39.5 (1.0) | 5.9 (1.5) | 1.5 (0.8) | 3.3 (1.1) | 14.2 (1.6) | 2.5 (1.6) | 51.6 (1.1) | 2.9 (0.7) | 7 (0.6) |
| PAO ΔdacC ΔpbpG | 55.9 (1.0) | 39.4 (1.0) | 4.6 (1.2) | 1.2 (0.6) | 3.3 (1.1) | 8.9 (1.0) | 9.8 (6.1) | 48.8 (1.1) | 2.6 (0.6) | 11.2 (1.0) |
| PAO ΔdacB ΔdacC ΔpbpG | 54.7 (1.0) | 40 (1.0) | 5.2 (1.3) | 1.3 (0.7) | 2.4 (0.8) | 7.6 (0.9) | 66.4 (41.5) | 50.6 (1.1) | 2.5 (0.6) | 13.2 (1.2) |
| PAO ΔampC | 59.9 (0.9) | 36.3 (1.0) | 3.8 (1.0) | 1.2 (0.6) | 3.3 (1.1) | 9 (1.0) | 2.5 (1.6) | 43.8 (1.0) | 2.8 (0.7) | 11.1 (1.0) |
| PAO ΔdacB ΔampC | 54.2 (1.0) | 40.3 (1.0) | 5.4 (1.4) | 2.1 (1.1) | 4.1 (1.3) | 10.3 (1.2) | 2.5 (1.6) | 51.2 (1.1) | 4.1 (1.0) | 9.8 (0.9) |
| PAO ΔdacC ΔampC | 59.7 (1.0) | 36.6 (1.0) | 3.7 (1.0) | 1.1 (0.6) | 3.2 (1.0) | 9.1 (1.0) | 8 (5.0) | 44.1 (1.0) | 2.4 (0.6) | 11 (1.0) |
| PAO ΔpbpG ΔampC | 56.4 (1.0) | 38.6 (1.0) | 4.9 (1.3) | 1.5 (0.8) | 3.7 (1.2) | 9.7 (1.1) | 2.5 (1.6) | 48.5 (1.1) | 3.1 (0.8) | 10.3 (0.9) |
| PAO ΔdacB ΔdacC ΔampC | 59.4 (1.0) | 36.1 (0.9) | 4.4 (1.1) | 1 (0.5) | 2.5 (0.8) | 7.9 (0.9) | 32 (20.0) | 45.2 (1.0) | 2 (0.5) | 12.6 (1.0) |
| PAO ΔdacB ΔpbpG ΔampC | 49.4 (0.9) | 43.5 (1.1) | 7 (1.8) | 1.7 (0.9) | 3.6 (1.2) | 11.3 (1.3) | 3.7 (2.3) | 57.8 (1.3) | 3 (0.7) | 8.8 (0.8) |
| PAO ΔdacC ΔpbpG ΔampC | 54.9 (1.0) | 39.9 (1.0) | 5.1 (1.3) | 1.8 (1.0) | 3.7 (119) | 9.4 (1.1) | 9.4 (5.9) | 50.4 (1.1) | 3.5 (0.9) | 10.7 (0.9) |
| PAO ΔdacB ΔdacC ΔpbpG ΔampC | 54.8 (1.0) | 40 (1.0) | 5.1 (1.3) | 0.6 (0.3) | 2.1 (68) | 7.2 (0.8) | 67.6 (42.2) | 50.4 (1.1) | 1.1 (0.3) | 13.9 (1.2) |
| PAO ΔdacB ΔpbpG ΔampC ΔdacC | 53 (0.9) | 40.4 (1.1) | 6.5 (1.7) | 1.5 (0.8) | 2.4 (77) | 8.8 (1.0) | 65.9 (41.2) | 53.6 (1.2) | 2.8 (0.7) | 11.4 (1.0) |
Mono, monomers; Di, dimers; Tri, trimers; D-D, muropeptides having Dap-Dap peptide bridges; Lpp, muropeptides bound to C-terminal Arg-Lys dipeptide of Braun's lipoprotein; Anh, muropeptides having anhydro-1,6-anhydromuramic acid; Penta, muropeptides having a pentapeptide stem. Values in parentheses represent the ratio of the values obtained for each mutant and wild-type PAO1.
Cross-link, degree of peptidoglycan cross-linking (percentage). Values in parentheses represent the ratio of the values obtained for each mutant and wild-type PAO1.
D-D/T, percent ratio of Dap-Dap cross-links to total peptidoglycan cross-links. Values in parentheses represent the ratio of the values obtained for each mutant and wild-type PAO1.
Values in parentheses represent the ratio of the values obtained for each mutant and wild-type PAO1.