TABLE 3.
HPLC analysis of muropeptides prepared from the peptidoglycan of the different PAO1 mutants with FOX treatment
| Strain or mutant | Relative abundance (mol%) of muropeptidea: |
Cross-linkb | D-D/Tc | Peptidoglycan lengthd | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mono | Di | Tri | D-D | Lpp | Anh | Penta | ||||
| PAO1 | 55.5 (1.0) | 39.3 (1.0) | 5.1 (1.3) | 1.9 (1.0) | 3.7 (1.2) | 10.1 (1.2) | 4.4 (2.8) | 49.9 (1.1) | 3.7 (0.9) | 9.9 (0.9) |
| PAO ΔdacB | 57.1 (1.0) | 37.2 (1.0) | 5.6 (1.2) | 1.7 (1.1) | 3.4 (0.9) | 13.7 (1.4) | 3.7 (1.5) | 48.6 (1.0) | 3.5 (1.1) | 7.3 (0.7) |
| PAO ΔdacC | 55.2 (0.9) | 39.6 (1.1) | 5.1 (1.4) | 1.2 (1.1) | 2.5 (0.8) | 8.2 (0.9) | 38.8 (5.5) | 50.1 (1.1) | 2.3 (1.0) | 12.3 (1.1) |
| PAO ΔpbpG | 56.6 (1.0) | 37.9 (1.0) | 5.4 (1.3) | 1.9 (1.3) | 3.6 (1.3) | 8.9 (1.0) | 4.2 (3.0) | 49.0 (1.1) | 3.9 (1.2) | 11.3 (1.1) |
| PAO ΔdacB ΔdacC | 58.8 (1.0) | 36.7 (1.0) | 4.4 (0.9) | 1.1 (1.0) | 3.2 (0.9) | 8.1 (0.9) | 39.8 (1.4) | 45.8 (1.0) | 2.4 (1.0) | 12.4 (1.2) |
| PAO ΔdacB ΔpbpG | 51.0 (0.9) | 42.1 (1.1) | 6.8 (1.2) | 2.1 (1.4) | 4.9 (1.5) | 12.3 (0.9) | 4.0 (1.6) | 56.1 (1.1) | 3.7 (1.3) | 8.2 (1.2) |
| PAO ΔdacC ΔpbpG | 54.3 (1.0) | 40.3 (1.0) | 5.3 (1.2) | 1.0 (0.8) | 2.6 (0.8) | 7.5 (0.8) | 44.0 (4.5) | 51.3 (1.1) | 1.8 (0.7) | 13.4 (1.2) |
| PAO ΔdacB ΔdacC ΔpbpG | 54.8 (1.0) | 39.0 (1.0) | 6.0 (1.2) | 1.1 (0.9) | 3.1 (1.3) | 9.3 (1.2) | 66.0 (1.0) | 51.6 (1.0) | 2.1 (0.8) | 10.8 (0.8) |
| PAO ΔampC | 54.9 (1.0) | 40.3 (1.1) | 4.6 (1.2) | 1.3 (1.1) | 2.6 (0.8) | 7.2 (0.8) | 14.0 (5.6) | 50.0 (1.1) | 2.6 (0.9) | 14.0 (1.3) |
| PAO ΔdacB ΔampC | 55.8 (1.0) | 39.5 (1.0) | 4.5 (0.8) | 1.3 (0.6) | 3.4 (0.8) | 7.7 (0.8) | 16.5 (6.6) | 48.9 (1.0) | 2.7 (0.7) | 13.1 (1.3) |
| PAO ΔdacC ΔampC | 58.1 (1.0) | 37.0 (1.0) | 4.8 (1.3) | 1.4 (1.3) | 3.4 (1.1) | 7.5 (0.8) | 62.3 (7.8) | 46.9 (1.1) | 3.0 (1.3) | 13.3 (1.2) |
| PAO ΔpbpG ΔampC | 53.9 (1.0) | 40.6 (1.1) | 5.4 (1.1) | 1.8 (1.2) | 3.1 (0.8) | 8.2 (0.9) | 14.1 (5.6) | 51.7 (1.1) | 3.5 (1.2) | 12.3 (1.2) |
| PAO ΔdacB ΔdacC ΔampC | 56.9 (1.0) | 37.6 (1.0) | 5.3 (1.2) | 1.2 (1.2) | 2.6 (1.0) | 7.5 (1.0) | 63.1 (2.0) | 48.8 (1.1) | 2.5 (1.3) | 13.3 (1.1) |
| PAO ΔdacB ΔpbpG ΔampC | 53.4 (1.1) | 40.6 (0.9) | 5.8 (0.8) | 1.9 (1.1) | 3.6 (1.0) | 8.3 (0.7) | 14.7 (4.0) | 52.8 (0.9) | 3.6 (1.2) | 12.0 (1.4) |
| PAO ΔdacC ΔpbpG ΔampC | 55.8 (1.0) | 38.5 (1.0) | 5.5 (1.1) | 1.3 (0.7) | 2.8 (0.8) | 7.3 (0.8) | 64.6 (6.9) | 50.2 (1.0) | 2.5 (0.7) | 13.7 (1.3) |
| PAO ΔdacB ΔdacC ΔpbpG ΔampC | 57.0 (1.0) | 37.2 (0.9) | 5.6 (1.1) | 1.3 (2.2) | 2.7 (1.3) | 6.7 (0.9) | 63.1 (0.9) | 49.1 (1.0) | 2.7 (2.3) | 15.0 (1.1) |
| PAO ΔdacB ΔpbpG ΔampC ΔdacC | 57.2 (1.1) | 36.7 (0.9) | 5.9 (0.9) | 1.0 (0.7) | 2.0 (0.8) | 7.4 (0.8) | 65.5 (1.0) | 49.0 (0.9) | 1.9 (0.7) | 13.5 (1.2) |
Mono, monomers; Di, dimers; Tri, trimers; D-D, muropeptides having Dap-Dap peptide bridges; Lpp, muropeptides bound to C-terminal Arg-Lys dipeptide of Braun's lipoprotein; Anh, muropeptides having anhydro-1,6-anhydromuramic acid; Penta, muropeptides having a pentapeptide stem.
Cross-link, degree of peptidoglycan cross-linking (percentage). Values in parentheses represent the ratio of the values obtained for each strain with and without cefoxitin exposure.
D-D/T, percent ratio of Dap-Dap cross-links to total peptidoglycan cross-links. Values in parentheses represent the ratio of the values obtained for each strain with and without cefoxitin exposure.
Values in parentheses represent the ratio of the values obtained for each strain with and without cefoxitin exposure.