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Production of melanin by yeast cells of Paracoccidioides spp. and analysis of phenoloxidase activity. (A and B) Light microscopy of ATCC 32069 growth on YPD medium supplemented with l-DOPA (A) and on YPD medium without l-DOPA (B). Original magnification, ×400. (C) Phenoloxidase activity of melanized Paracoccidioides spp. compared with C. albicans and C. neoformans. UE, units of enzyme. *, statistically significant difference (P < 0.0001). The error bars indicate SEM.
