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MTT assay and ROS production after treatment with itraconazole and amphotericin B. (A and B) Metabolic activities of nonmelanized and melanized yeast cells treated with itraconazole (A) and amphotericin B (B) at the MIC. (C) ROS production after treatment with itraconazole and amphotericin B at the MIC. *, statistically significant difference in comparison to nonmelanized cells (P < 0.0001). mel−, nonmelanized cells; mel+, melanized cells. The error bars indicate SEM.
