TABLE 2.
Groups and conditions used for determination of the optimal TBO concentration and LED light dose for in vitro antimicrobial photodynamic inhibition of Paracoccidioides spp.
| Group | Conditions |
|---|---|
| TBO concna | |
| Control groups | |
| C1 | Untreated |
| C2 | Irradiated by LED with a light dose of 90 J/cm2 without a photosensitizer |
| C3 | Exposed to TBO at a concn of 10 mg/liter without light irradiation |
| C4 | Exposed to TBO at a concn of 40 mg/liter without light irradiation |
| C5 | Exposed to TBO at a concn of 70 mg/liter without light irradiation |
| Treatment groups | |
| T1 | Exposed to TBO at a concn of 10 mg/liter followed by LED irradiation with a light dose of 90 J/cm2 |
| T2 | Exposed to TBO at a concn of 40 mg/liter followed by LED irradiation with a light dose of 90 J/cm2 |
| T3 | Exposed to TBO at a concn of 70 mg/liter followed by LED irradiation with a light dose of 90 J/cm2 |
| LED light dosea | |
| Control groups | |
| C1 | Untreated |
| C2 | Exposed to TBO at a concn of 10 mg/liter without light emission |
| C3 | Irradiated with LED at a dose of 30 J/cm2 without photosensitizer exposure |
| C4 | Irradiated with LED at a dose of 60 J/cm2 without photosensitizer exposure |
| C5 | Irradiated with LED at a dose of 90 J/cm2 without photosensitizer exposure |
| Treatment groups | |
| T1 | Exposed to TBO at a concn of 10 mg/liter and then LED irradiated with a light dose of 30 J/cm2 |
| T2 | Exposed to TBO at a concn of 10 mg/liter and then LED irradiated with a light dose of 60 J/cm2 |
| T3 | Exposed to TBO at a concn of 10 mg/liter and then LED irradiated with a light dose of 90 J/cm2 |
a
Five-minute preincubation of the fungal conidia with TBO was performed in the dark.