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DapNS strains have less aconitase activity than DapS parental strains. Aconitase activities were determined in triplicate in at least three independent cultures after 6 h (postexponential phase) of growth. The results presented are averages, and error bars represent the standard deviations. The statistical significance of differences between a DapS strain and the corresponding DapNS strain, determined by Student's t test, is indicated (*, P < 0.1; **, P < 0.05; ***, P < 0.005).
