FIG 4.

Significantly altered metabolites of Dap^NS strain 703 compared to those of parental Dap^S strain 616. Metabolite concentration changes were identified by 2D ¹H-¹³C HSQC NMR analyses in triplicate of bacteria grown aerobically in TSB medium containing [¹³C]glucose. Metabolites are highlighted according to their chemical nature as amino acids (white), amino sugar/sugars/sugar alcohol (black), pyrimidines/purines (dark gray), or others (bright gray). Positive values represent higher concentrations, and negative values represent lower concentrations, of the metabolites in the Dap^NS strain than those of the metabolites in the Dap^S strain. Abbreviations: GlcNAc, N-acetyl-d-glucosamine; Ery-4-phosphate, erythrose-4-phophate; AXP, AMP, ADP, or ATP; CXP, CMP, CDP, or CTP; dA/dG, deoxyadenosine/deoxyguanosine. Each bar is shown with the statistical significance determined by Student's t test at the 90% (*, P < 0.1) or 95% (**, P < 0.05) confidence level.