FIG 4.

Cytopathic effects of the combinations with autophagy inhibitors on human corneal epithelial cells. To reduce the cytopathic effects of PHMB on the HCE cells, PHMB was diluted from 0.02% to 0.000625% and the diluted PHMB was used for incubation with HCE cells for 2 h. (A) The lowest concentrations of PHMB, 0.00125% and 0.000625%, did not show the cytopathic effects on the HCE cells. (B to D) The cytopathic effects of PHMB were not increased with the addition of 3MA, wortmannin, or chloroquine. The data are expressed as means ± SEM of the results of three independent experiments, performed in triplicate.