TABLE 1.
PCR primers used to assess the genetic context of β-lactamase genes
| Primer name | Sequence | Length (bp) | Target | Reference or source |
|---|---|---|---|---|
| CMY2_F | GCTGAGAGCTCATGATGAAAAAATCG | 1,146 | blaCMY-2 | This study |
| CMY2_R | GGTACGGATCCTTATTGCAGC | |||
| Primers for blaCMY-2 genetic environment determination | ||||
| TraA-F | ATCAGTTGGCGAATGCCTCA | 1,417 | traA-ISEcp1 | This study |
| ISEcp1-R | AACACGGCTTCATTCGCCCAA | This study | ||
| ISEcp1-F | ATAAAGACCATGCTCTGCGG | 2,537 | ISEcp1- blaCMY-2 | This study |
| CMY-2R | ACGGACAGGGTTAGGATA | 15 | ||
| ISEcp1-F | ATAAAGACCATGCTCTGCGG | 2,800 | ISEcp1- blaCMY-2-blc | This study |
| blc-R | TACCAGGTTCCCAGATAGCG | This study | ||
| ISEcp1-F(B) | ATAAAGACCATGCTCTGCGG | 3,465 | ISEcp1- blaCMY-2-blc-sugE | This study |
| sugE-R(B) | TTGGCCTGAAATACACCCAC | This study | ||
| A-F | CGTAGAGGATCTCAGTTCAG | 3,700 | traB-traV-traA- ISEcp1- blaCMY-2 | 22 |
| A-R | CCTGCCACTGTTTGCCTGTC | |||
| G-F | CGCCTGCTAAAAGCAAGAAT | 2,700 | sugE-ecnR-orf1 | 22 |
| G-R | TGCCATCTGCATGAACAAAT | |||
| H-F | AAGGCTGTTGGCTTCGAGTA | 2,500 | orf1-orf2-orf3 | 22 |
| H-R | TCAGCAGTATCACCCTGCAC | |||
| I-F | ATGTGTTCAAAGCCGAAACC | 2,500 | orf3-orf4-dsbC-traC | 22 |
| I-R | GGTGCCGTAATCGAAGATTT | |||
| Primers for blaPER-1-positive plasmid characterization | ||||
| PER1-F | GCTCCGATAATGAAAGCGT | 520 | blaPER-1 | 15 |
| PER1-R | TTCGGCTTGACTCGGCTGA | |||
| TEM1-F | CATTTCCGTGTCGCCCTTATTC | 800 | blaTEM-1 | 15 |
| TEM1-R | CGTTCATCCATAGTTGCCTGAC | |||
| ISCR1-F | AGACGCCGTGGAAGCGTGTG | 509 | ISCR1 | This study |
| ISCR1-R | GCTCGCTCACACCCTCAGCC | |||
| QnrVC6-F | ATGGAAAAATCAAAGCAATT | 657 | qnrVC6 | 5 |
| QnrVC6-R | TTAGTCAGGAACAATGAT | |||
| Int1-F | GGCATCCAAGCAGCAAGC | Various | Gene cassette (class I integron) | 25 |
| Int1-R | AAGCAGACTTGACCTGAT |