FIG 1.

Effect of antibiotic treatment on initiation of C. difficile spore germination. C. difficile UK1 spores were suspended in BHIS-Ca⁺⁺ medium (black) or medium supplemented with surotomycin at 8× MIC (A) or 80× MIC (B) or vancomycin or metronidazole at 8× MIC (C) or 80× MIC (D). Where indicated, the medium was supplemented with taurocholic acid (TA), an activator of germination. The initiation of spore germination was detected by measuring the loss of OD₆₀₀. Experiments were performed in duplicate; the presented data represent one such experiment. Germination plots from the two experiments were superimposable.