FIG 4.

Romidepsin treatment leads to the upregulation of select ISG transcription. PBMCs were primed with the indicated concentration of HDACi for either 2 h (left) or 8 h (right). After priming, the cells were analyzed using a Fluidigm multiplex gene array. The cells were primed with recombinant interferon beta (rIFN-β) as a positive control for altered gene expression. The fold changes in transcription relative to baseline are presented in each box of the grid. In the heat map, yellow hues indicate stable transcription relative to baseline, green hues indicate reduced transcription relative to baseline, and red hues indicate increased transcription relative to the baseline. Representative data from one of two human donors are shown. Arrows indicate the fold changes consistently above 1.5.