TABLE 3.

Gene enrichment analysis of P. aeruginosa biofilms compared to planktonic cells based on Gene Ontology groups (43, 44)

Gene Ontology term	Gene count	P value
Upexpressed in biofilms
Nitrogenous compound/oxidoreductase activity	8	2.3 × 10−10
Heme binding	16	1.2 × 10−8
Heme-copper terminal oxidase activity	5	1.8 × 10−3
Organic acid catabolic processes	6	3.2 × 10−3
Downexpressed in biofilms
Nitrogen compound biosynthetic processes	107	9.0 × 10−29
Translation	71	1.3 × 10−42
tRNA aminoacylation	20	3.0 × 10−12
Nucleotide biosynthesis	39	7.7 × 10−16
Tricarboxylic acid cycle	12	5.8 × 10−6
Histidine biosynthesis	11	1.2 × 10−7
NADH dehydrogenase activity	12	3.4 × 10−8
GTP binding	15	3.6 × 10−6
RNA processing	24	3.5 × 10−4
Lipid biosynthetic processes	37	1.6 × 10−9
Aspartate family amino acid biosynthesis	14	4.8 × 10−6
Glutamine family amino acid biosynthesis	18	2.2 × 10−4
Nucleotide kinase activity	5	2.0 × 10−4
DNA topoisomerase activity	5	7.1 × 10−5
Glycolysis	10	5.0 × 10−5
Coenzyme/folic acid metabolic processes	33	2.8 × 10−7
Metal ion binding	99	7.6 × 10−4
Alcohol biosynthetic processes	7	1.2 × 10−4
Purine biosynthetic processes	23	2.1 × 10−9
Protein folding	11	3.3 × 10−3
Aromatic amino acid biosynthesis	16	9.9 × 10−4
Fatty acid biosynthesis	11	3.3 × 10−3
Cellular respiration	20	7.2 × 10−5
Pyrimidine biosynthetic processes	9	4.4 × 10−5
Nucleoside metabolic processes	14	5.4 × 10−4
DNA replication	1	5.7 × 10−3
Protein transmembrane transport	5	2.3 × 10−3