TABLE 4.

MICs of actinomycin D, arylomycin A-C₁₆, and deoxycholic acid for Gram-negative strains determined at 37, 28, and 20°C

Temp (°C)	Strain	MIC (μg/ml)
		Actinomycin D	Arylomycin A-C16	Deoxycholic acid
37	E. coli MG1655	>64	>64	>50,000
	Y. enterocolitica ATCC 23715	64	>64	>50,000
	Y. pestis KIM6+	4	4	6,250
	Y. pestis KIM6+ LepB(P91S) (DBS600)	4	0.03	6,250
	E. coli lptD4213 (BAS901)	0.06	16	500
	E. coli LepB(P84L) (PAS0260)	64	2	>50,000
	Y. pestis KIM6+ ΔphoP	8	16	25,000
	Y. pestis KIM5	8	4	3,100
	Y. pestis KIM5-pLpxL	16	8	25,000
	Y. pestis KIM6+ LepB(P91S) Δail (DBS601)	16	1	12,500
28	Y. enterocolitica ATCC 23715	64	>128	>50,000
	Y. pestis KIM6+	32	16	50,000
	Y. pestis KIM6+ LepB(P91S) (DBS600)	32	0.06	50,000
	E. coli lptD4213 (BAS901)	−a	16	−
	E. coli LepB(P84L) (PAS0260)	>64	2	>50,000
	Y. pestis KIM6+ ΔphoP	32	>64	50,000
	Y. pestis KIM5	>64	16	50,000
	Y. pestis KIM5-pLpxL	>64	16	50,000
	Y. pestis KIM6+ LepB(P91S)Δail (DBS601)	32	1	50,000
20	Y. pestis KIM6+	>64	>64	50,000
	Y. pestis KIM6+ LepB(P91S) (DBS600)	−	1	−
	E. coli lptD4213 (BAS901)	−	16	−
	E. coli LepB(P84L) (PAS0260)	>64	2	>50,000
	Y. pestis KIM6+ ΔphoP	>64	>64	50,000
	Y. pestis KIM5	32	>64	50,000
	Y. pestis KIM5-pLpxL	−	>64	−
	Y. pestis KIM6+ LepB(P91S)Δail (DBS601)	64	1	50,000

^a−, not determined.