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. 2015 Jun 16;5:11196. doi: 10.1038/srep11196

Figure 2. Detection of GAD65-GADA immune complexes using solid-phase PLA.

Figure 2

A) GAD65-GADA complexes was detected similarly to the measurement of GAD65 using solid-phase PLA, but replacing one of the PLA probes specific for GAD65 with one directed against human IgG 1. B) Solid-phase PLA was used to measure GAD65-GADA immune complexes in blood serum samples from SPS patients (n = 7) and control samples (n = 13). Significantly increased levels of GAD65-GADA complexes were recorded in the SPS group (median number of PCR starting amplicons 155 (red bar)) in comparison with the control group (median No. of PCR starting amplicons 10 (blue bar)) (p = 0.002). C) Scatter plot showed the correlation between two independent measurements of GAD65-GADA complexes as calculated numbers of starting PCR amplicons in the SPS group (R2 = 0.99, red circles) and the control group (R2 = 0.49, blue diamonds). D) Scatter plot presenting the correlation between measurements of GAD65-GADA complexes and GAD65 in samples from SPS patients (R2 = 0.96).