Table 1.
Gene mutation status of BRAF, PIK3CA, PTEN, and hTERT and protein expression of PTEN and pAKT in the melanoma cell line panel.
| Cell line | Mutation status |
Protein expression |
||||
|---|---|---|---|---|---|---|
| BRAF | PIK3CA | PTEN | hTERT | PTEN | pAKT | |
| NZM3 | V600K | WT | WT | WT | +++ | +/− |
| NZM6 | V600E | WT | Exon 3 deletion | WT | − | +++ |
| NZM7 | V600E | WT | WT | C250T | +++ | ++ |
| NZM11 | V600E | WT | WT | WT | +++ | +/− |
| NZM12 | V600E | WT | WT | C250T | +++ | ++ |
| NZM20 | V600E | WT | WT | C228T | +++ | +/− |
| NZM30 | V600E | WT | WT (exons 2–9) Exon 1 unknown | C228T | − | +++ |
| NZM34 | V600E | WT | Exon 5 frameshift | C250T | − | ++ |
| NZM43 | V600K | WT | Exon 1 frameshift | WT | − | ++ |
WT, wild type; +++, high expression; ++, moderate expression; +, low expression; +/−, not expressed in some blots and low expression in others; −, not expressed in all blots.