Skip to main content
. 2015 Jun 16;9:223. doi: 10.3389/fncel.2015.00223

Figure 1.

Figure 1

Disruption of Slc4a10 increases somatic field potentials in the hippocampus. (A,B) Representative examples of fEPSPs recorded in the stratum radiatum (A) and stratum pyramidale (B) in WT and KO slices upon stimulation of Schaffer collaterals with increasing stimulus intensity (0–60 V), respectively. (C) Slopes of fEPSPs recorded in the stratum radiatum of CA1 are independent of Slc4a10 (p > 0.05; n = 20/19). (D) CA1 population spike amplitudes upon stimulation of Schaffer collaterals were increased in acute brain slices of Slc4a10 KO mice (p = 0.04; n = 22/27). (E) The correlation between the slope of fEPSP recorded in the stratum radiatum and the amplitude of PS in the stratum pyramidale revealed a significant increase in spike coupling (p < 0.001, n = 14/14). (F) Exemplary traces of fEPSP recorded in the stratum radiatum (F) upon repeated stimulation of Schaffer collaterals with an inter-stimulus interval of 120 ms in WT and KO slices. (G) Disruption of Slc4a10 had no impact on dendritic paired-pulse facilitation (p > 0.05; n = 18/17). (H) Mean fEPSP slopes upon stimulation with half-maximal stimulus intensity did not differ between genotypes (p > 0.05; n = 18/17). (I) Exemplary responses of fEPSP recorded in the stratum pyramidale upon repeated stimulation of Schaffer collaterals with an inter-stimulus interval of 120 ms in WT and KO slices. (J) Paired-pulse facilitation at an inter-stimulus interval of 80 and 120 ms was decreased (p = 0.04; n = 18/22). (K) Note, at half-maximal stimulus intensity the mean first PS amplitude also showed a significant increase in KO slices. (p = 0.001; n = 18/22). *p < 0.05 **p < 0.005 ***p < 0.001.