Figure 4.

Small molecule inhibition of MRP1 and chemoresponse in Recurrent GBM tumor derived cell lines. Treatment of recurrent tumor-derived GBM cell lines, MZ-304, and MZ-256, led to a significant increase in both vincristine and etoposide-induced cell death (n = 3 ^*p < 0.05, ^**p<0.01, ^***p < 0.001 Unpaired Student T-test) (A) compared to chemotherapy alone treatment. The presence of MK571 (25 μM) did not have any effect on temozolomide-induced cell death in either of these cell lines. Treatment with Reversan (15 μM), significantly increased cell death for all three chemotherapies tested (n = 3 ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 Unpaired Student T-test) in both lines assessed (B).