Figure 3.

Neutrophil influx is increased in the absence of mast cells. Twenty-four hours pi, cornea pairs from WT and Kit^W-sh mice were harvested and digested in type I collagenase. Single cell suspensions were labeled with antigen-specific monoclonal antibodies and gated to identify CD45^hi total leukocytes (a, b); F4/80⁻ Gr-1⁺ PMN (a, d3); F4/80⁺ Gr-1⁻ macrophages (b, d1); F4/80⁺ Gr-1⁺ inflammatory monocytes (b, d2); and CD3⁻ NK1.1⁺ NK cells by flow cytometry. Representative histograms for CD45 expression (overlay: dark gray = Kit^W-sh 24 hours pi, black = WT 24 hours pi, light gray = UI WT) and dot plots for F4/80 and Gr-1 expression are presented in (c) and (d), respectively. Relative frequencies of these populations are shown in the dot plots. Data reflect two independent experiments (n = 5 mice/group) and is shown as mean ± SEM.