FIGURE 1.

Small protein B (SmpB) enhanced enhanced green fluorescent protein (eGFP) production under the control of bvgS promoter. (A) Schematic representation of the constructs. The promoter and initial nine-residue of N-terminal BvgS were fused to eGFP, and introduced between pBR322 origin and ampicillin resistance gene to generate pDH114 by restriction sites Eag I and Bgl II. The native promoter and smpB gene, the native smpB promoter, the native promoter and encoded gene of GST were inserted between tetracyclin resistant gene and p15A origin to produce pDH212, pDH213, pDH214 using Nco I and Xho I, respectively. (B) Time courses of relative fluorescence in Escherichia coli ΔtmRNA-smpB co-transformed pDH114 with pDH212, pDH213, and pDH214, separately. The fluorescence of cells was measured and normalized to corresponding OD₆₀₀. Error bars represented the SD of three independent cultures. (C) Column graph comparing the relative fluorescence at 24 h. (D) Time courses of relative fluorescence in E. coli two-hybrid reporter strains co-transformed pDH114 with pBT and pBT-SmpB, separately. The strains were induced by 1 mM IPTG at 37°C compared with the uninduced ones. (E) Column graph comparing the relative fluorescence in pBT and pBT-SmpB after 24 h IPTG treatments.