Methods |
Study design: controlled before-and-after study Study location: Kotmale oya, below Kotmale dam, Sri Lanka Study dates: May to August 2000 Transmission intensity: epidemic Malaria parasite species: not specified Primary vectors: An. culicifacies adanensis (national importance), An. annularis, An. subpictus, An. tessellatus (local importance) Breeding sites: pools formed in riverbed between dam and power plant Baseline data: one day before stocking |
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Participants |
Not applicable |
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Interventions |
Fish species: P. reticulata Indigenous fish species used: no Fish source: riverbed pools below the Kotmale dam and then reared in stock tanks at Regional Office Anti-Malaria Campaign, Kandy Populated sites: 60 riverbed pools, 0.25 to 1.0 m2 surface area and < 1 m depth (29 experimental, 31 control, randomly selected) Restocked: no Co-interventions: none |
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Outcomes |
Number (percentage) of pools positive for anopheline larvae Mean number of larvae per pool Mean number of larvae per 100 dips Method: larval dipping using 100 mL dipper, six dips per m2. Authors collected anopheline immatures but reported larval numbers only |
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Source of funding |
National Research Council, Sri Lanka (NRC Grant No. 99/09) |
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Notes |
Fish number monitored An. culicifacies not identified at any sites |
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Risk of bias |
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Bias |
Authors' judgement |
Support for judgement |
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Study design |
High risk |
Controlled before-and-after study |
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Site selection |
Unclear risk |
"Sixty isolated riverbed pools...were selected and labeled" |
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Site allocation |
Unclear risk |
"P. reticulata was stocked in 29 randomly selected pools". Method of randomization not described |
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Blinding of outcome assessment (detection bias) All outcomes |
High risk |
"Visual counts of P. reticulata were made in each pool, monthly. Visual counts were possible, as the pools were small (not exceeding 1 m2 surface area), shallow (< 1 m depth) and contained clean water" |
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Baseline values |
Low risk |
Comparable between control and experimental sites |
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Number of sites |
Low risk |
Adequate numbers of sites in control (31 site) and experimental groups (29 sites) |