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. 2013 Dec 10;(12):1–65. doi: 10.1002/14651858.CD008090.pub2

Kusumawathie 2008a

Methods Study design: controlled before-and-after study Study location: Kotmale oya, below Kotmale dam, Sri Lanka Study dates: May to August 2000 Transmission intensity: epidemic Malaria parasite species: not specified Primary vectors: An. culicifacies adanensis (national importance), An. annularis, An. subpictus, An. tessellatus (local importance) Breeding sites: pools formed in riverbed between dam and power plant Baseline data: one day before stocking

Participants Not applicable

Interventions Fish species: P. reticulata Indigenous fish species used: no Fish source: riverbed pools below the Kotmale dam and then reared in stock tanks at Regional Office Anti-Malaria Campaign, Kandy Populated sites: 60 riverbed pools, 0.25 to 1.0 m2 surface area and < 1 m depth (29 experimental, 31 control, randomly selected) Restocked: no Co-interventions: none

Outcomes Number (percentage) of pools positive for anopheline larvae Mean number of larvae per pool Mean number of larvae per 100 dips Method: larval dipping using 100 mL dipper, six dips per m2. Authors collected anopheline immatures but reported larval numbers only

Source of funding National Research Council, Sri Lanka (NRC Grant No. 99/09)

Notes Fish number monitored An. culicifacies not identified at any sites

Risk of bias

Bias Authors' judgement Support for judgement

Study design High risk Controlled before-and-after study

Site selection Unclear risk "Sixty isolated riverbed pools...were selected and labeled"

Site allocation Unclear risk "P. reticulata was stocked in 29 randomly selected pools". Method of randomization not described

Blinding of outcome assessment (detection bias) All outcomes High risk "Visual counts of P. reticulata were made in each pool, monthly. Visual counts were possible, as the pools were small (not exceeding 1 m2 surface area), shallow (< 1 m depth) and contained clean water"

Baseline values Low risk Comparable between control and experimental sites

Number of sites Low risk Adequate numbers of sites in control (31 site) and experimental groups (29 sites)