Sabatinelli 1991

Methods	Study design: controlled interrupted time series study Study location: Grande Comore Island, Federal Islamic Republic of Comoros Study dates: November 1987 to November 1988 Transmission intensity: endemic Malaria parasite species: not specified Primary vectors: An. gambiae Breeding sites: domestic water containers Baseline data: November 1987
Participants	Not applicable
Interventions	Fish species: P. reticulata Indigenous fish species used: not indigenous Fish source: imported from Mayotte Island Populated sites: domestic water containers; 20 unstocked (ablution basins) for duration of trial; 59 ablution basins and 61 tanks stocked in November 1987. Stocking of basins and tanks extended, and by April 1988, all basins and tanks were treated. Total numbers of basins and tanks stocked not specified Restocked: not clearly indicated Co-interventions: temephos (concentration: 2 cc/m3) in tanks only, last treatment March 1988
Outcomes	Percentage of containers positive for anopheline larvae Method: Surface and bottom of containers were examined for An. gambiae larvae (at least 15 cm in diameter), which were recorded monthly
Source of funding	Research was undertaken with the framework of project OMS-PNUD COM/MAL/001
Notes	No environmental data collected
Risk of bias
Bias	Authors' judgement	Support for judgement
Study design	High risk	Controlled interrupted time series study
Site selection	Unclear risk	Unclear how sites were selected
Site allocation	Unclear risk	Unclear how experimental treatment was selected. Control sites were in village of Bandamadji 3 km from experimental site
Blinding of outcome assessment (detection bias) All outcomes	Unclear risk	Unclear whether assessors were blinded to treatment
Baseline values	Low risk	Percentage of sites positive for An. gambiae larvae comparable in control and experimental groups
Number of sites	Low risk	Adequate numbers of sites in control and experimental groups