De Zulueta 1961 UGA.

Methods	Dates of study: 1959‐1960 Location of study: Uganda Malaria endemicity (prevalence): 34% in children 2‐9 years; 17% in all ages [Moderate] Transmission season: Rainy season April to May, August to November Malaria species: P. falciparum, P. malariae Vector species: A. gambiae, A. funestus Study design: Uncontrolled before‐and‐after study Evaluation design: Cross‐sectional surveys
Participants	Age groups included: All ages Sample size Intervention group 1 mean (range): 30,384 (10,303 ‐ 59,605)
Interventions	Intervention group 1 (North Kigezi): MDA administered to all persons with chloroquine 600 mg and pyrimethamine 50 mg every three months for four rounds at the time of IRS application from May 1959 to May 1960. Coverage 80%. Co‐intervention with IRS.
Outcomes	Parasitaemia prevalence Gametocytaemia prevalence No adverse event surveillance conducted No adverse events reported
Notes	Outcomes assessed in a sub‐sample of the treated population.
Risk of bias
Bias	Authors' judgement	Support for judgement
Random sequence generation (selection bias)	High risk	No comparison group
Allocation concealment (selection bias)	High risk	No comparison group
Baseline imbalance (selection bias)	High risk	No comparison group
Contamination protection	High risk	No comparison group
Blinding of participants and personnel (performance bias) All outcomes	High risk	No comparison group
Blinding of outcome assessment (detection bias) All outcomes	High risk	No comparison group
Incomplete outcome data (attrition bias) All outcomes	Low risk	Cooperation of the local inhabitants was remarkably good and not a single dwelling was left unsprayed
Selective reporting (reporting bias)	Low risk	Increased number of samples from hyperendemic areas in the post‐intervention survey
Other bias	Low risk	No other bias detected