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. 2015 Jun 11;9:2947–2959. doi: 10.2147/DDDT.S79475

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© 2015 Zhang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Construction of the recombinant bispecific VEGF-ephrin A1-PE38 immunotoxin used in this study.

Notes: (A) Schematic drawing of the adenoviral vector pAdTrack-CMV encoding the VEGF₁₆₅-ephrin A1-PE38 fusion-toxin gene fragment. The positions of the restriction-enzyme digestion sites are marked. (B) The plasmid was identified by agarose-gel electrophoresis (1%) after restriction-enzyme digestion. “1” represents the blank pAdTrack-GV218 vector. “2” represents the gene fragment VEGF₁₆₅-ephrin A1-PE38.

Abbreviations: CMV, cytomegalovirus; GBM, glioblastoma multiforme.