Fig. 1.

Intrathecal poly I:C induced IFN-β in the CNS. a IFN-β reporter mice received poly I:C by intrathecal injection and luciferase activity was visualized after 4 and 24 h. Poly I:C induced IFN-β in the brain and spinal cord at 4 and 24 h. IFN-β response was much stronger at 4 h than at 24 h. Bar graphs depict the quantification of luciferase activity at indicated time points (n = 4). Data were analyzed by two-tailed nonparametric Student’s t test followed by Mann–Whitney test. Results are presented as mean ± SEM. *P < 0.05. Distribution of IFN-β-producing cells in the CNS. IFN-β/YFP+ staining (green) was observed in meninges around brain (b, c) and spinal cord (d) as well as choroid plexus (e), as indicated by red boxes on the central schematic. Nuclei were stained with DAPI (blue). Scale bars 50 µm (b–e). CP choroid plexus in fourth ventricle, LC leptomeningeal cortex, LM leptomeningeal midbrain, LVMF leptomeningeal ventral median fissure. Original magnification 10×