Fig. 2.

Cellular sources of IFN-β in the CNS. a–c Double immunostaining showed that IFN-β/YFP+ was induced in cells (green) that co-localized both with CD45 (red) and d with Mac1/CD11b (red), in mice treated with poly I:C. e Higher magnification of double positive YFP+ Mac1/CD11b+ cells in d (white box, magnification 20×). f Poly I:C-induced IFN-β/YFP+ (green) stained cells within meninges did not co-localize with GFAP+ staining (red). DAPI (blue) shows nuclear staining. g–i IFN-β/YFP+ staining was not induced in CNS from a PBS-treated mouse. j IFN-β/YFP+ staining was absent in sections incubated with normal rabbit antibody. Scale bars 50 µm (a–d, g–j) and 20 µm (e, f). Original magnification was 10×